Lee et al. demonstrated that eupatilin activates the mitochondrial apoptotic pathway in osteosarcoma cell lines (U-2 OS) through an increase in the Bax/Bcl-2 ratio, a decrease in mitochondrial membrane potential, cytochrome c release, and caspase-3/9 activation and PARP cleavage [45]. The gene discussed is CYCS; the disease is osteosarcoma.