This study presents the GMP-compliant production of a novel PDGFRβ-targeted PET radiopharmaceutical, [68Ga]Ga-DOTA-Z09591 ([68Ga]Ga-ATH001), and its preclinical evaluation in mouse and human myocardial tissue, along with initial clinical imaging in patients with ST-elevation myocardial infarction (STEMI). Here, PDGFRB is linked to ST Elevation Myocardial Infarction.