Overall, 58.7% (152/259) of the canine blood samples assessed were positive for at least one canine TBP following PCR, including Ehrlichia (40.5%: 105/259) and Babesia (17.4%: 45/259), with 7.3% (19/259) exhibiting co-infection with both Ehrlichia and Babesia species. This evidence concerns the gene TBP and coinfection.