The AUC of GPC3 in diagnosing AFP-negative HCC was 0.681 (95% CI: 0.595–0.758, p = 0.0005), and the AUC of DCP in diagnosing AFP-negative HCC was 0.783 (95% CI: 0.704–0.849, p = 0.0005). Here, GPC3 is linked to hepatocellular carcinoma.