In this context, either PTX loaded in cyclodextrin-based nanosponges [9] or ICOS-Fc loaded in polymer nanoparticles (PLGA or cyclodextrin-based nanoparticles) [18] are highly effective in vivo against the non-BRAF mutated B16-F10 melanoma, which is less aggressive than the D4M-3A model used in the present work. The gene discussed is BRAF; the disease is melanoma.