In addition, the Western blot assay of small intestine on D 7 illustrated a noticeable reduction in ZO−1 levels in the infection group that significantly improved gut barrier integrity by reducing intestinal permeability by PMC105 treatment rather than the treatment group, which is evidenced by the high level of tight junction protein ZO−1 in the treated mice group (Fig. 3F). This evidence concerns the gene TJP1 and infection.