EGFR and Hyperglycemia: Immunofluorescence showed an increased expression of CD274 with hyperglycemia, and this is supported by the fact that hyperglycemia can increase the expression of CD274 (PD-L1), often by altering cellular metabolism and activating specific signaling pathways, including epidermal growth factor receptor (EGFR), which activates downstream pathways like Ras-Extracellular signal-regulated kinase (RAS/ERK) [40].