Proteomic and RNA analyses have consistently shown that the cargo of seminal EVs differs between fertile men and those with asthenozoospermia, with specific candidates (e.g., TRPV6 levels in spEVs) correlating with calcium regulation and motility characteristics; region-specific EVs along the tract display unique marker constellations and biophysical signatures, underscoring the importance of distinguishing and reporting EV subtypes rather than combining them [26]. Here, TRPV6 is linked to Reduced sperm motility.