ERBB2 and neoplasm: Figure 3 illustrates a representative example of a high-level HER2 amplification pattern, characterized by clustered black signals within the tumor cell nuclei. This finding confirms gene amplification and supports the classification of the tumor as HER2-positive, making the patient eligible for targeted anti-HER2 therapy, in accordance with ASCO/CAP guidelines. In contrast, Figure 4 presents a case without HER2 gene amplification, confirmed by DISH analysis.