Subsequent studies using B16-F10 melanoma cells further revealed SFN-induced apoptosis marked by caspase-3 and -9 activation, upregulation of Bax and p53, and suppression of anti-apoptotic markers such as Bcl-2, Bid, NF-κB, and caspase-8 [23]. The gene discussed is BCL2; the disease is melanoma.