SOX2 and neoplasm: To assess whether polyphenol treatments could influence the stem cell properties of ATC cells in a 3D context, the gene expression of stemness markers (NANOG and SOX2) and differentiation markers (SOX17) was assessed after treatment with RSV, 3-MET-OX, and ISOR-H-PG in spheroids of ATC cell lines and non-tumor controls (Figure 5).