TNF and allergic asthma: Similarly, calycosin can interact with GPER to increase occludin expression, improve E-cadherin distribution, and inhibit TSLP production, contributing to protecting the epithelial barrier in allergic asthma treatments at a dose of 10 mg/kg in a house dust mite (HDM)-induced allergic asthma mouse model, and at the effective concentration of 10 μM in the TNF-α- and Poly (I:C)-co-stimulated human bronchial epithelial cell line [147].