AKT1 and osteosarcoma: By contrast, calycosin within the concentration range of 25–100 μM can induce the apoptosis of ER+ MG-63 human osteosarcoma cells by increasing the expression of proteins related to the PI3K/Akt/mTOR pathway; meanwhile, it has no significant effect on ER-U2-OS cells, indicating its selective action on ER+ cells [174].