Calycosin within the concentration range of 25–100 μM can increase the apoptosis rate in human ovarian carcinoma SKOV3 cells by up-regulating the ratio of Bax/Bcl-2 and increasing the expressions of caspase proteins, such as caspase-9 and caspase-3, in which Bax is a pro-apoptotic protein, while Bcl-2 is an anti-apoptotic protein. Here, BAX is linked to ovarian carcinoma.