Methods: This study utilized a nested case–control cohort of longitudinal pre-diagnostic serum samples from the UK Collaborative Trial of Ovarian Cancer Screening (UKCTOCS) profiled for eight candidate ovarian cancer biomarkers (CA125, HE4, PEBP4, CHI3L1, FSTL1, AGR2, SLPI, DNAH17) and 92 additional cancer-associated proteins from the Olink Oncology II panel. This evidence concerns the gene FSTL1 and ovarian carcinoma.