TGFB1 and familial pancreatic carcinoma: For pancreatic cancer, it has two prominent technical advantages: first, it confirms the core role of epigenetic regulation in subtype differentiation with a data integration efficiency of 93%; second, it can capture the time lag between epigenetic changes and gene expression, such as the 48-h advance of the TGF-β/Smad3 pathway in epigenetic regulation before corresponding gene expression changes.