GCLC and nutritional deficiency disease: Although glutamate-cysteine ligase catalytic subunit (GCLC) is induced in livers of rats fed ethanol [76], its expression, along with that of glutathione synthetase (GS), the second enzyme in GSH synthesis, is decreased by 50% in livers of AH patients [60], suggesting that the decrease in hepatic GSH levels in ALD is likely multifactorial, including nutritional deficiency, decreased precursor availability (SAMe), and decreased capacity to synthesize GSH.