To further validate the actual expression levels of the five diagnostic genes in endometriosis and RIF, we performed immunofluorescence staining and Western blot analysis on endometrial tissues from normal, endometriosis, and RIF groups.Immunofluorescence double staining showed that the fluorescence signal intensities of SRPRB, RBM3, INSIG2, GYG1, and FBXW2 (red fluorescence) were significantly decreased in both disease groups compared to the normal group, while Vimentin expression (green fluorescence) was also generally reduced in endometriosis and RIF groups (Fig. 7A,C,E,G,I). The gene discussed is GYG1; the disease is endometriosis.