Cell clusters were annotated based on canonical markers from established literature [31]: tumor cells (SOX2+SOX9+GFAP+), M1-like MDM (CD68+P2Y12−CD163−), M2-like MDM (CD68+P2Y12−CD163+), M1-like MG (CD68+P2Y12+CD163−), M2-like MG (CD68+P2Y12+CD163+), T cells (CD68−CD3+), monocytes (CD14+ and/or CD16+), endothelial cells (CD31+). The gene discussed is GFAP; the disease is neoplasm.