IDH1 and astrocytoma (excluding glioblastoma): Notably, these immune interactions were significantly enriched in high-grade IDH-mutant astrocytoma (Supplementary Fig. 3E) compared to their low-grade counterparts (Supplementary Fig. 3F), suggesting cluster 7 may play a key role in modulating the immune microenvironment through crosstalk with T cells and macrophages.