GPT and liver disorder: Additionally, miR-224-5p showed the closest clustering with G/S grading, and correlation analysis also revealed that miR-224-5p expression displayed the most significantly positive correlation with G grade (r=0.55), S stage (r=0.60), ALT (r=0.41), AST (r=0.48), and AFP (r=0.36) levels, suggesting that miR-224-5p may play a critical role in hepatic inflammatory responses and the progression of advanced liver diseases.