Rotenone enhanced inflammation & retinal detachment, while DCA alleviated EAU.Rotenone promoted microglial activation, while DCA decreased it.LPS+IFN-γ strengthened microglial migration and proliferation.YY1 de-lactylation alleviated microglial migration and proliferation.Rotenone enhanced the expression of inflammatory cytokines (iNOS, COX-2, TNF-α), where DCA improved this effect. The gene discussed is TNF; the disease is retinal detachment.