Subpopulation specific KEGG analysis indicated that Mac_Adam8 exhibited activation of ferroptosis, TNF signaling, apoptosis, NOD-like receptor signaling, and lysosomal pathways, along with Salmonella and Legionella infection pathways, but inhibition of osteoclast differentiation and endocytosis (Figure 5C, p < 0.05). The gene discussed is TNF; the disease is legionellosis.