In the present study, the one-bag group (S1) demonstrated a slower resolution of acidemia, a prolonged duration of insulin infusion, and an increased incidence of hypoglycemia, likely secondary to variability in prescriber practice, apart from the lack of the use of the two-bag method. These findings necessitated the reevaluation of the DKA treatment guidelines at our institution, especially with regards to fluid administration and insulin therapy. This evidence concerns the gene INS and Hypoglycemia.