Long-term antibiotic treatment leads to significant alterations in gut microbiota and leads to osteoporosis, and that localized RAS in bone tissue promotes osteoclast proliferation and activity through up-regulation of ERK1/2 to promote RANKL release and inflammatory infiltration, leading to osteoporosis, and that the angiotensin II Type 2 Receptor (AT2R) may be an important target for this process. This evidence concerns the gene MAPK3 and osteoporosis.