However, principal component analysis (PCA), as well as the rare cell analysis tools CellSIUS, EDGE, and scCAD [50], failed to enrich the SRCC population (e.g., the SLC52A2+NOTCH1+ population) from 208,506 cells or 11,763 cancer cells harboring copy number variation (CNV) on uniform manifold approximation and projection plots (Fig. 4A, left panel, Fig. S4A–F, Table S6). This evidence concerns the gene NOTCH1 and cancer.