SH2D3A and infection: Following high-dose infection with CHIKV-Nluc, reporter activity was similar across T-REx-U2OS, T-REx-U2OS-CHIKV nsP1, and T-REx-U2OS-RRV nsP1 cells, indicating that expression of exogenous CHIKV or RRV nsP1 had no apparent impact on the translation or replication of the wt CHIKV genome.