Under low-dose infection conditions, SFV nsP1 failed to restore replication of CHIKV nsP1Y248A-Nluc entirely (Fig 5D) indicating that the complementation provided by SFV nsP1 is relatively weak and, under low-dose infection conditions, is outweighed by its inhibitory effect on CHIKV replication. The gene discussed is SH2D3A; the disease is infection.