Figure 4E clearly shows that, following treatment with SLNT at the same concentration, the viability of CD133+ CRC cells were significantly reduced compared to that of their CD133- counterparts. Above results explained that CRC cells with high levels of CD133 are more susceptible to the cytotoxic effects of SLNT. Notably, CD133+ colorectal cancer cells are well-recognized to possess stemness properties. Therefore, the results presented in Figure 4E further support that SLNT exerts a potent stemness-inhibitory effect on CRC cells. This evidence concerns the gene PROM1 and colorectal cancer.