Here, we successfully constructed stably transduced CD133-knockdown HCT116 cells (shCD133 HCT116 cells) and CD133-overexpressing SW480 cells (OECD133 SW480 cells), and reported that CD133 promoted the growth and proliferation of CRC cell lines both in vitro (Figures 4A,B). Here, PROM1 is linked to colorectal carcinoma.