Non-fasted serum insulin at time of harvest was increased in mCH-CH-WD mice compared with all other groups (Supplementary Figure S4B), whereas serum glucose levels were increased in mWD-CH-WD mice (Supplementary Figure S4C), potentially indicating that mWD with WD challenge in adulthood predisposed these mice to the insulin resistance that is often seen in juvenile obesity (34, 35). The gene discussed is INS; the disease is Insulin resistance.