INS and Wilson disease: Non-fasted serum insulin at time of harvest was increased in mCH-CH-WD mice compared with all other groups (Supplementary Figure S4B), whereas serum glucose levels were increased in mWD-CH-WD mice (Supplementary Figure S4C), potentially indicating that mWD with WD challenge in adulthood predisposed these mice to the insulin resistance that is often seen in juvenile obesity (34, 35).