- Elevated nucleotide metabolism in comparison with normal epithelial cells- Elevated EGFR, hypoxia, MAPK and TGF-β signaling in tumor regions- The hub gene for nucleotide metabolism NME1 (identified through sc-seq) suppresses migration and stemness in vitro and predicts immunotherapy response in CRC patients. The gene discussed is NME1; the disease is colorectal carcinoma.