ESR1 and breast cancer: In recent years, studies have found that tumor necrosis factor-alpha (TNF-α) in PBMCs of breast cancer patients and non-cancer individuals; interleukins 6 and 8 (IL-6 and IL-8),the mRNA expression of estrogen receptor alpha (ER-α) was different, and based on ROC curve analysis, the diagnostic performance of IL-6 was AUC = 0.825 (95% CI: 0.549–0.94, P = 0.030), indicating that breast cancer can be screened and diagnosed earlier by detecting the expression of various influencing factors in PBMCs in peripheral blood (Zare Moayedi et al., 2021).