In recent years, studies have found that tumor necrosis factor-alpha (TNF-α) in PBMCs of breast cancer patients and non-cancer individuals; interleukins 6 and 8 (IL-6 and IL-8),the mRNA expression of estrogen receptor alpha (ER-α) was different, and based on ROC curve analysis, the diagnostic performance of IL-6 was AUC = 0.825 (95% CI: 0.549–0.94, P = 0.030), indicating that breast cancer can be screened and diagnosed earlier by detecting the expression of various influencing factors in PBMCs in peripheral blood (Zare Moayedi et al., 2021). Here, ESR1 is linked to cancer.