Finally, RT-PCR results showed that the expression of PPAR γ mRNA, FAS mRNA, and fatty acid-binding protein 4 (FABP 4) mRNA was increased in the HF group compared with NC (P<0.05), whereas the expression of PPAR γ mRNA was decreased after 1,25(OH)2D3 treatment (P<0.05) (Figures 4H–J). Here, FAS is linked to hydrops fetalis.