PLIN2 and steatosis: In HF/HSD-fed AEG-1fl/fl mice, PLIN2 staining unraveled significantly high levels of microvesicular and macrovesicular steatosis and 100% large hepatocellular ballooning (HB) with strong intensity in male mice and 35% small to 25% medium HB with moderate intensity in female mice (Fig. 1, D and E).