We next analyzed the response of AEG-1-deleted KCs, and consequently of hepatocytes and other nonparenchymal cells, to HF/HSD feeding by performing single-cell RNA-Seq (scRNA-Seq) in livers from HF/HSD-fed AEG-1fl/fl and AEG-1ΔMAC littermates. This evidence concerns the gene TBCE and hydrops fetalis.