This multi-pronged approach, paracellular (TJ modulation), transcellular (enhanced endocytosis), and direct bypass (pineal gland fenestrae), explains the superior in vivo glioma targeting of MeB NPs, which achieved significantly higher fluorescence intensity in the glioma region compared to the transferrin-modified nanoparticles [142]. The gene discussed is TF; the disease is central nervous system cancer.