SRF was overexpressed by pcDNA-SRF plasmid and suppressed by CCG-1423 (a small molecule inhibitor of SRF) or SRF siRNA to study how SRF influences renal tubular EMyT through Cyr61 in the chronic progression after AKI.<h4>Results</h4>In TCMK-1 cells under H/R treatment and renal medulla tissue from I/R rats, the SRF along with Cyr61, collagen-3, α-SMA and vimentin expression was upregulated, while E-cadherin and ZO-1 expression was downregulated. The gene discussed is TJP1; the disease is acute kidney injury.