However, aligning with our previous ELISA results, mRNA levels of IL6 in Yersinia-infected or TNF-stimulated cells and CXCL8, for bacterial infection were significantly higher in C3-expressing cells in comparison to C3-deficient cells and similar trend was observed for treatments with Pam3CSK4 and TNF (Fig. 4A, B). Here, CXCL8 is linked to bacterial infectious disease.