In this synergistic setting, the frequency of IFN-γ+ and TNF-α+ CD4+ T cells in the SFB+ B16-3340 group showed extremely strong negative correlations with tumor burden (r = -0.9057 and r = -0.9742, respectively), indicating a direct linear relationship between Th1 responses and tumor eradication. The gene discussed is IFNG; the disease is neoplasm.