Western blot analysis of sEVs derived from NB cells confirmed MYCN-enrichment in sEVs isolated from MYCN amplified NB cell lines, such as KellyCis83, Kelly, SK-N-BE(2), and NB-1691 sEVs, in contrast to the non-MYCN-amplified SH-SY5Y and SK-N-AS sEVs (Figure 6E, Supplementary Figure S2, Supplementary Table S3). This evidence concerns the gene MYCN and neuroblastoma.