By combining behavioral assays sensitive to anxiety and exploratory activity—such as the elevated plus maze, open field test, and home-cage monitoring in the PhenoTyper® system—with targeted RT-qPCR analyses, we aimed to identify Negr1-dependent transcriptional responses within key limbic and cortical regions, particularly the prefrontal cortex and hippocampus. The gene discussed is NEGR1; the disease is Anxiety.