To further determine the regulation of Ifn-λr1 by Plscr1 in response to Ifn-λ signaling without the complexities associated with live virus infection, high molecular weight poly(I:C) was administered intranasally to mice daily for 6 days at 2.5 mg/kg of body weight, and mice were sacrificed on the following day (Figure 3—figure supplement 4A). Here, IFNLR1 is linked to viral infectious disease.