PADI4 and alkaline phosphatase measurement: Moreover, qRT-PCR analyzes of pancreatic tissues confirmed significant upregulation of inflammatory cytokine genes (Il6, Tnfa, Il1b), chemokines (Cxcl1, Cxcl2), and key NET-associated genes (Padi4, Nfkb, Nlrp3, Il17a) in the FMT-AP group (Figure 4I).