,33 Moreover, the up-regulation of LRRC8A can increase the formation and secretion of extracellular vesicles (EVs) in colorectal cancer cells, and LRRC8A is concomitantly excreted along with the extracellular vesicle contents into the extracellular environment, where extracellular vesicle-bound LRRC8A acts as a regulator to preserve the stability of the extracellular vesicle volume in response to alterations in extracellular osmotic pressure.34 This evidence concerns the gene LRRC8A and colorectal cancer.