A cluster-by-cluster pathway enrichment analysis demonstrated that these inferred genes are involved in a wide array of crucial biological processes, including cell cycle and proliferation (regulation of gene expression, cell cycle, and centromere/kinetochore function), signaling pathways (nuclear receptor signaling, AMPK, PI3K-Akt, Rho GTPase, and VEGF signaling), cellular structure and specialization (myogenesis, keratinization, and actin cytoskeleton organization), metabolism & disease (insulin signaling, glycogen metabolism, cataract formation, and Hirschsprung disease). Here, VEGFA is linked to Hirschsprung disease.