In this study, we evaluate the diagnostic accuracy and feasibility of a novel point-of-care test (POCT) in predicting LVO stroke, along with the levels of vascular biomarkers—NT-proBNP, D-Dimer, and H-FABP—from ultra-early whole-blood samples of patients with suspected stroke in a prehospital setting. The gene discussed is NPPB; the disease is stroke disorder.