To further elucidate the role of NK cells and its link to IFN signaling in anti-MDA5+ DM-ILD pathogenesis, we performed single-cell RNA sequencing (scRNA-seq) analysis on peripheral blood mononuclear cells (PBMCs) from patients with anti-MDA5+DM with and without ILD. This evidence concerns the gene IFNA1 and dermatomyositis.