Then, we investigated the alteration of each subtype between Untreated group and ICB-treating group (Figure 4C), consistent with our spatial profiling results, PD-1+TRM cells were found to be significantly enriched in tumor parenchyma; in contrast, the abundance of other CD8+ T cell subtypes did not differ substantially across tissue compartments, and by comparing the gene expression of each subtype of CD8+T cell, we identified PCNA was enriched in the PD-1+TRM (Figure 4D), In line with the result of the increasing proportion of PD-1+TRM in ICB-treating group. The gene discussed is CD8A; the disease is neoplasm.