Subsequent clustering analysis, combined with marker-based annotation, allowed us to define the major cell populations, including T/NK cells, B/plasma cells, myeloid cells, endothelial cells, epithelial cells, and fibroblasts (Figure 3C), considering T cells play an important role in anti-tumor immunity, we applied type-specific markers that categorized T cells into CD4+T, CD8+T and NK cells aimed to compare the percentage of different subtypes (Figure 3D). This evidence concerns the gene CD4 and neoplasm.