Nanobody-161 had antitumor activity in a transgenic mouse model, reducing tumor weight by 5-fold at a dose of 7.5 mg/kg and inhibited TNFα-TNFR2 signaling in HEK293 cells overexpressing human TNFR2 with 10-fold greater potency than traditional antagonists. Here, TNFRSF1B is linked to neoplasm.