,18 To investigate the effect of SENP1 on CSC-associated properties in HCC, overexpression and silencing approaches were respectively applied to HCC cell lines with low (Huh7 and PLC5) and high (HepG2 and Hep3B) endogenous SENP1 expression (Fig. S1). Here, SENP1 is linked to hepatocellular carcinoma.