KL and Myocardial fibrosis: Secondly, it activates the Nrf2 signaling pathway, up-regulates the expression of antioxidant enzymes, improves oxidative stress and mitochondrial function, and reduces the accumulation of reactive oxygen species (ROS); at the same time, Klotho can also reduce myocardial fibrosis and collagen deposition by inhibiting TGF-β/Smad and Wnt/β-catenin signaling pathways.