Similar findings were obtained in cocultures with HLA-A24+ RCAN1-1pos RCAN1-4neg GBM cells (SF10281), although to a lesser extent (Supplementary Fig. 6D–F), indicating that RCAN1-422-32 can induce an antitumor T cell response even in the context of low RCAN1-4 expression. This evidence concerns the gene RCAN1 and glioblastoma.