Importantly, CD8+ T cells primed with RCAN1-422-32, but not RCAN1-177-87, secreted high levels of IFN-γ when cocultured with HLA-A24+ RCAN1-1pos RCAN1-4pos GBM cells (SF10360) (Fig. 3E‒G, Supplementary Fig. 6A‒C). This evidence concerns the gene CD8A and glioblastoma.