Furthermore, both the flow cytometry and immunofluorescence staining results confirmed that MSLN×CD16A could specifically bind to MSLN-positive tumor cells (MKN45, NCI-N87, HGC27, and CFPAC-1) but did not bind to MSLN-negative tumor cells (MiaPaCa-2 and HuH-7) (Fig. 2e-i, Supplementary Fig. S1d). Here, MSLN is linked to neoplasm.