The outliers on significant genes in Figure 2 were all located, relative to the regression line, in a position consistent with an extremely aged individual based on our observations of slowing of epigenetic ageing with CR; additionally, the 4 CpG sites for Wasf3 and the 5 sites for Zic1 show the outliers to be hypo‐ and hypermethylated respectively, consistent with published HCC biomarkers (Supporting Information Table S3, Supporting Information Figure S2). This evidence concerns the gene ZIC1 and hepatocellular carcinoma.