To assess the impact of EV treatment on the phagocytic profile of activated microglia in the AD brain, we quantified the expression of lysosomal protein CD68 in conjunction with the pan‐microglial marker IBA1 (ionized calcium‐binding adaptor molecule) using dual immunofluorescence staining and 3D algorithm‐based volumetric quantification of co‐localized, immunoreactive surfaces. The gene discussed is AIF1; the disease is Alzheimer disease.